` Sample Pre-Processing Development
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Technology Development

Sample Pre-Processing Development

Our technology development aim is focused on automation of sample pre-processing for mass spectrometry-based metabolomics. This will allow for integration of sample pre-processing platform into field deployable sample collection devices. Currently metabolomics sample pre-processing is time consuming and prone to human error due to sample handling procedures. Our goal is to take advantage of fabric phase solid extraction (FPSE) techniques, which are compatible with downstream ultra-performance liquid chromatography time of flight mass spectrometry (UPLC-ToFMS) metabolomics. To this end we have tested different fabric phase extraction protocols in collaboration with the Sample Engineering Core to capture a full metabolomic and lipidomic profile in blood. Our initial experiment identified a material, which is efficient in extracting polar metabolites and lipid species from different sample types; whole blood, serum, and diluted serum. Current efforts are focused on refining our protocols to optimize extraction efficiency while using just 50 uL of sample each time.




Columbia University




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Center for High-Throughput Minimally-Invasive Radiation Biodosimetry