While untargeted metabolomics focuses on global changes and profiling, targeted metabolomics assesses only specific metabolites or pathways. Additional benefits include, with enrichment of samples, to quantify molecules with extremely low concentrations in biological samples. A targeted metabolomics approach was undertaken to assess changes in the serum of irradiated mice.
The AbsoluteIDQTM p180 kit allowed for the identification of a large number of statistically significant ions, belonging in pathways and categories depicted in the pie chart.
With the targeted oxylipins method we were to identify and quantify the changes in specific categories of low concentration lipids and show for the first time a significant shift from an anti-inflammatory (omega-3) to a pro-inflammatory (omega 6) phenotype following exposure to ionizing radiation.