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The Micronucleus Assay

The Cytokinesis Blocked Micornucleus assay (CBMN) quantifies radiation-induced chromosome damage expressed as post-mitotic micronuclei, in once-divided cells. For the CBMN assay we use the standard Fenech scoring criteria.

A new binarization routine was developed specifically for processing images of DAPI-stained lymphocytes, where staining intensity of micronuclei is often lower than that of the main nuclei. We have seen excellent agreement between the yield of automatically scored micronuclei and those scored manually from the same images, also up to 8 Gy, although the yield of Micronuclei saturates above 5 Gy, probably due to mitotic delay.

A flow of the Micronucleus assay as implemented in the RABiT is shown below:

 

 

Links

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Columbia University

Georgetown

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Center for High-Throughput Minimally-Invasive Radiation Biodosimetry